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GenPlex¢â v1.0 is the professional software for analysis that is developed to analyze the DNA chip data. In v1.0, it focused on the analysis of two-dye chip data (cDNA microarray, two-dye long oligoarray etc). Here are the GenPlex¢â distinct special features: It modulated each part independently by systemizing all of chip data analysis processes into 4 steps that are Preprocessing, DEG finding (Differentially Expressed Gene), Clustering and Classification. Thus, it enabled the user to recognize each step of analysis clearly. Moreover, it considered analysis of relation between modules and allowed the user to locate it from the results window of each module to other modules. Thus, it is well designed the independence of main analysis and interconnection. Preprocessing Module- Input data by using the wizard method and focused on simplicity of data filtering. - Global normalization, Lowess normalization (arraywise/blockwise) - Embodied Global normalization, Lowess normalization (arraywise/blockwise) DEG Module- Embodied fold change and various kinds of statistic algorithms (Welch¡¯s t-test, one-way ANOVA etc) - Contains the volcano plot that can compare the fold change and the results of statistic test intuitively. - Able to understand the distribution of each sample group in three-dimension by using the sample PCA. Clustering Module- Embodied main clustering algorithm (hierarchical clustering, K-means, SOM) - Provide various linkage method of Hierarchical clustering - Excellent visual abilities such as dendrogram, topographic 2D cluster profile etc. - Applied statistical clustering validation method to find the most appropriate clustering method. Classification Module- It systemized the whole process of classification into feature selection, classifier and error estimation parts, and embodied the whole computation function that can perform the whole process at once. - Embodied the widely used method in the feature selection (BSS/WSS, regularized t-test, Kruskal-Wallis test etc). - Embodied the widely used methods in the classifier (weighted KNN, prototype matching, FLDA etc). - Embodied the widely used method in error estimation (complete/incomplete LOOCV, bootstrap etc). |